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  "Title": "Estimates Allele Frequency on qPCR DeltaDeltaCq from Bulk\nSamples",
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  "Date/Publication": "2022-01-26 10:54:10 UTC",
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      "title": "Introduction to freqpcr",
      "author": "Masaaki Sudo",
      "engine": "knitr::rmarkdown",
      "headings": [
        "Masaaki Sudo",
        "External links",
        "TL;DR",
        "Model structure",
        "Modeling allelic DNA content in a mixed sample solution consisting of multiple individuals",
        "Uncertainty of individual DNA yield: approximation by gamma and beta distributions",
        "Measurement of sample allele frequency using qPCR: ΔΔCq method and RED-ΔΔCq method",
        "RED-ΔΔCq method",
        "ΔΔCq method by qPCR using allele-specific primer sets",
        "Calculating sample allele frequency on ΔCq and $\\delta_{\\mathrm{T}}$",
        "Interval estimation of population allele frequency based on multiple bulk samples",
        "Diploidy",
        "freqpcr assuming continuous DNA quantities (eDNA)",
        "Dependency",
        "Installation",
        "Usage",
        "Variables and parameters",
        "Estimation of auxiliary experimental parameters with knownqpcr() or knownqpcr_unpaired()",
        "Dummy Cq data generation",
        "knownqpcr() on RED-ΔΔCq dataset",
        "knownqpcr() on general ΔΔCq dataset",
        "Estimation with knownqpcr_unpaired()",
        "Estimation of population allele frequency using freqpcr()",
        "Assign the estimated parameter values to R variables",
        "Create a dummy Cq dataset for running freqpcr() demo",
        "Estimation with freqpcr()",
        "freqpcr() on ΔCq analysis",
        "Fixing parameters for freqpcr()",
        "Misc",
        "Required sample size",
        "To circumvent frequent missing values of target1",
        "Citation",
        "Package author",
        "License",
        "History",
        "Referenes"
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      "created": "2021-12-21 21:52:21",
      "modified": "2022-01-25 12:41:53",
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