Introduction to freqpcr5 years ago
Masaaki Sudo | External links | TL;DR | Model structure | Modeling allelic DNA content in a mixed sample solution consisting of multiple individuals | Uncertainty of individual DNA yield: approximation by gamma and beta distributions | Measurement of sample allele frequency using qPCR: ΔΔCq method and RED-ΔΔCq method | RED-ΔΔCq method | ΔΔCq method by qPCR using allele-specific primer sets | Calculating sample allele frequency on ΔCq and $\delta_{\mathrm{T}}$ | Interval estimation of population allele frequency based on multiple bulk samples | Diploidy | freqpcr assuming continuous DNA quantities (eDNA) | Dependency | Installation | Usage | Variables and parameters | Estimation of auxiliary experimental parameters with knownqpcr() or knownqpcr_unpaired() | Dummy Cq data generation | knownqpcr() on RED-ΔΔCq dataset | knownqpcr() on general ΔΔCq dataset | Estimation with knownqpcr_unpaired() | Estimation of population allele frequency using freqpcr() | Assign the estimated parameter values to R variables | Create a dummy Cq dataset for running freqpcr() demo | Estimation with freqpcr() | freqpcr() on ΔCq analysis | Fixing parameters for freqpcr() | Misc | Required sample size | To circumvent frequent missing values of target1 | Citation | Package author | License | History | Referenes